USP20 antibody

Catalog No. ABIN7120505

The Rabbit Polyclonal anti-USP20 antibody has been validated for ELISA, WB, IHC and IP. It is suitable to detect USP20 in samples from Human, Mouse and Rat.

Quick Overview for USP20 antibody (ABIN7120505)

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP20 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-USP20 Antibody

Purpose: USP20 antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: ubiquitin specific peptidase 20

Isotype: IgG

Application Details

Application Notes: WB: 1:200-1:2000, IHC: 1:20-1:200,IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for USP20

Target: USP20 (Ubiquitin Specific Peptidase 20 (USP20))

Alternative Name: USP20

Background:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 20|Deubiquitinating enzyme 20|Ubiquitin thioesterase 20|Ubiquitin-specific-processing protease 20|VHL-interacting deubiquitinating enzyme 2 (hVDU2)|USP20|KIAA1003|LSFR3A|VDU2

Background: Deubiquitinating enzyme involved in beta-2 adrenergic receptor(ADRB2) recycling. Acts as a regulator of G-protein coupled receptor(GPCR) signaling by mediating the deubiquitination beta-2 adrenergic receptor(ADRB2). Plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2. Upon dissociation, it is probably transferred to the translocated beta-arrestins, possibly leading to beta-arrestins deubiquitination and disengagement from ADRB2. This suggests the existence of a dynamic exchange between the ADRB2 and beta-arrestins. Deubiquitinates DIO2, thereby regulating thyroid hormone regulation. Deubiquitinates HIF1A, leading to stabilize HIF1A and enhance HIF1A-mediated activity. Mediates deubiquitination of both 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains.

Molecular Weight: 110 kDa

Gene ID: 10868

UniProt: Q9Y2K6

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling